FIELD: medicine.
SUBSTANCE: invention relates to medicine, namely to a method for sperm treatment. The method for sperm treatment includes: coloring of a sperm sample containing viable sperm carrying X-chromosome and viable sperm carrying Y-chromosome by a coloring medium; bringing of the colored sperm sample into contact with flowing liquid in a flow; and manipulation of a ratio of viable sperm carrying X-chromosome to viable sperm carrying Y-chromosome to form at least one sperm population subjected to manipulation. In this case, one or more of flowing liquid and coloring medium contains cryoprotector in a concentration from 0.1% to 6% vol./vol. or wt./vol.
EFFECT: above-described method allows for an increase in sperm motility after thawing and improvement of its sorting, namely improvement of the efficiency, throughput and rate.
16 cl, 37 dwg, 18 tbl, 17 ex
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