APPARATUS AND METHODS FOR HIGH-THROUGHPUT SEMEN COLLECTION Russian patent published in 2020 - IPC B01L3/02 A61B17/43 G01N15/14 

FIELD: biotechnology.

SUBSTANCE: disclosed are a system, a microfluidic chip and a semen sorting method. System includes sample source, substrate, flow channel with sample inlet channel, geometry of central jet formation, verification area with electromagnetic radiation source, detector, analyzer and collection tank. Chip includes a substrate and flow channels, besides, each flow channel includes an inlet channel, a geometry of formation of the central jet, a check area, and also outlet channels. Geometry of formation of central jet includes area of side focusing of fluid medium, a first channel for vertical focusing of fluid medium and a second channel for vertical focusing of fluid medium. First and second channels have a loop-like shape. Method comprises directing sperm through flow channels, supplying protective fluid medium into flow channel, deflection of the first part of protective fluid medium from the flow channel and repeated introduction of the first part of protective fluid medium in the first place, deflection of the second part of protective fluid medium from the flow channel and repeated introduction of the second part of the protective fluid medium in the second place, orientation of the sperm inside the flow channels, Orientation of the oriented sperm through the test region, sperm examination, differentiated orientation of the sperm from undirected sperm and selection of the targeted sperm subpopulation.

EFFECT: inventions provide favorable sorting conditions for sperm.

139 cl, 68 dwg