FIELD: biochemistry, in particular protein isolation from marrow cells of animal tubular bones.
SUBSTANCE: claimed method includes marrow cell isolation, suspending thereof, suspension filtration, filtrate centrifugation. Further precipitates together with supernatants in centrifugal test-tubes are triply frozen and thawed in refrigerated room, filtered through nylon-6 filter and precipitated with 10 % trichloroacetic acid solution at 60°C, settled for 12 h at 4°C and centrifuged at 1700 rpm for 5 min. Protein solution is further subjected to dialysis with flowing water for 1-2 days and distilled water for 1 hour.
EFFECT: simplified method; target product of improved quality.
3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR RECOVERING PROTEINS FROM BONE MARROW OF ANIMALS | 2019 |
|
RU2726615C1 |
| METHOD OF EXTRACTING PROTEINS FROM BONE MARROW CELLS | 2013 |
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| METHOD OF OBTAINING SUBSTANCE, POSSESSING ANTIMICROBIAL, ANTIVIRAL AND IMMUNOSTIMULATING ACTIVITY, IN PARTICULAR, WITH RESPECT TO DENDRITE CELLS, SUBSTANCE, THEREOF OBTAINED, AND BASED ON IT PHARMACEUTICAL COMPOSITION | 2013 |
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| AGENT FOR ACCELERATED REPAIRING OF STRUCTURE AND FUNCTION OF DAMAGED TISSUES AND ORGANS | 2004 |
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