METHOD FOR PROTEIN ISOLATION FROM MARROW CELLS Russian patent published in 2007 - IPC A23J1/10 A61K35/28 

FIELD: biochemistry, in particular protein isolation from marrow cells of animal tubular bones.

SUBSTANCE: claimed method includes marrow cell isolation, suspending thereof, suspension filtration, filtrate centrifugation. Further precipitates together with supernatants in centrifugal test-tubes are triply frozen and thawed in refrigerated room, filtered through nylon-6 filter and precipitated with 10 % trichloroacetic acid solution at 60°C, settled for 12 h at 4°C and centrifuged at 1700 rpm for 5 min. Protein solution is further subjected to dialysis with flowing water for 1-2 days and distilled water for 1 hour.

EFFECT: simplified method; target product of improved quality.

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