FIELD: biotechnology.
SUBSTANCE: method for mixing waste from the pharmaceutical processing of deer antlers with artificial gastric juice in a ratio of 1:10, keeping in a thermostat at a temperature of 40-45°C for four days, filtration of the supernatant, separation of polypeptides using 10% trichloroacetic acid in a ratio of 1:4, centrifugation at 3000 rpm for 5 min, separation of oligopeptides from the supernatant using 1.5% trichloroacetic acid solution, centrifugation at 3000 rpm for 5 min, adding 0.9% sodium chloride solution to the sediment, dialysis with running water for a day and distilled water for 1 h, centrifugation at 3000 rpm for 5 min, separation of the precipitate from solution, drying the precipitate in an oven at a temperature of 40-45°C, tyndallization.
EFFECT: increasing the yield of concentrated peptides in the form of a dry substance with a high purity of the target product.
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Authors
Dates
2023-08-22—Published
2023-02-27—Filed