METHOD FOR ISOLATING PEPTIDES FROM THE HYDROLYZATE OF WASTE FROM THE PHARMACEUTICAL PROCESSING OF DEER ANTLERS Russian patent published in 2023 - IPC A61K35/32 

Abstract RU 2802057 C1

FIELD: biotechnology.

SUBSTANCE: method for mixing waste from the pharmaceutical processing of deer antlers with artificial gastric juice in a ratio of 1:10, keeping in a thermostat at a temperature of 40-45°C for four days, filtration of the supernatant, separation of polypeptides using 10% trichloroacetic acid in a ratio of 1:4, centrifugation at 3000 rpm for 5 min, separation of oligopeptides from the supernatant using 1.5% trichloroacetic acid solution, centrifugation at 3000 rpm for 5 min, adding 0.9% sodium chloride solution to the sediment, dialysis with running water for a day and distilled water for 1 h, centrifugation at 3000 rpm for 5 min, separation of the precipitate from solution, drying the precipitate in an oven at a temperature of 40-45°C, tyndallization.

EFFECT: increasing the yield of concentrated peptides in the form of a dry substance with a high purity of the target product.

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RU 2 802 057 C1

Authors

Litvinova Zoia Aleksandrovna

Mandro Nikolai Mikhailovich

Dates

2023-08-22Published

2023-02-27Filed