METHOD OF EXTRACTING PROTEINS FROM BONE MARROW CELLS Russian patent published in 2015 - IPC A23J1/10 A61K35/28 

FIELD: chemistry.

SUBSTANCE: invention relates to biochemistry. Method includes extraction of bone marrow cells, their suspension, suspension filtration and centrifugation of filtrate. Sediments are subjected to homogenisation together with supernatant liquids. Then, content of centrifugal vials is filtered through polycaprolactam filters. Proteins of solution salted out by means of ammonium sulphate at heating to 60°C, settled for 12 h at temperature 4°C and centrifuged at 2000 rev/min for 5 min. 1% solution of trichloracetic acid is added to supernatant liquid, then sediment is also separated from solution by centrifugation at 2000 rev/min for 5 min. Obtained sediments are washed away with alcohol and ether and dried on watch glass 0.9% of sodium chloride solution is added to obtained powder, mixed thoroughly, then protein solution is subjected to dialysis with running water for 1-2 days and distilled water for 1 h. Solution is centrifuged at 2000 rev/min for 5 min, obtained sediments are washed away with alcohol and ether, dried on watch glass.

EFFECT: invention ensures simplification of method of general protein extraction, increase of target product purity, reduction of protein extraction time, application of novel technologies, availability, because bone marrow cells are taken from agricultural and wild animals.

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