FIELD: biochemistry, molecular biology.
SUBSTANCE: invention proposes a method for carrying out PCR in the presence of primers comprising of 3'-site that is complementary to a sequence-target and 5'-site that is not complementary to a sequence-target at temperature corresponding to the potential temperature of hybridization of primers along the whole length. Using the invention provides effective and highly specific amplification of nucleic acid. Invention can be used in molecular-genetic methods used in detection of nucleic acids.
EFFECT: improved method of PCR.
5 dwg
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