FIELD: biotechnology.
SUBSTANCE: method of carrying out PCR for efficient identification of allelic variants of Waxy-genes of wheat is described. The method differs from the known ones from the technical level in using the forward primer 4F-c: 5'-CCCCCAAGAGCAACTACCAGT-3'. Also the method of PCR-RFLP for efficient identification of allelic variants of Waxy-genes of wheat is described. The method differs from the known ones from the technical level in that after the step of PCR the procedure of RFLP-analysis is performed with endonuclease cleavage of amplicons by the restriction enzyme AcsI.
EFFECT: development of methods of carrying out PCR and PCR-RFLP for efficient identification of allelic variants of Waxy-genes of wheat.
2 cl, 4 dwg, 2 tbl
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