FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology and genetics of livestock. Method is proposed for carrying out real time PCR for genotyping cattle for alleles A and B of the CSN3 gene, in which used 5'-fluorescently-labeled direct A-allele-specific primer CSN3-A: 5'-FAM-ACTGTAGCTACTCTAGACGA-3', 5'fluorescently-labeled direct B-allele-specific primer CSN3-B: 5'-VIC-ACTGTAGCTACTCTAGATGC-3', reverse common primer CSN3-R: 5'-GCTCTCAATAACTTCTGGAGAA-3', anti-primer CSN3-S, labeled with a fluorescence quencher with 3'end of oligonucleotide: 5'-TCTAGAGTAGCTACAGT-3'-BHQ1.
EFFECT: invention makes it possible to efficiently carry out the genotyping of cattle by the CSN3 gene based on real time PCR in the format of hybridization-fluorescence detection.
1 cl, 2 dwg, 1 tbl
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