FIELD: biotechnology, genetic engineering, enzymology, biochemistry. SUBSTANCE: DNA fragment encoding the complete amino acid sequence of L-asparaginase from Erwinia caratovora (ECAR-LANS) is isolated and characterized. Plasmid DNA pBADLANS is constructed wherein enzyme-encoding DNA fragment is under control of regulated promotor pBAD. As result of transformation of E. coli cells with proposed recombinant plasmid and the following selection of transformed clones the new strain is prepared: producer ECAR-LANS (E. coli VKPM = B-8174) providing high yield of recombinant enzyme. The main designation of this enzyme is it using as the main component in preparing the known and development of new antitumor preparations. EFFECT: improved preparing method, valuable medicinal properties of enzyme. 2 cl, 7 dwg, 4 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| RECOMBINANT PLASMID DNA PACYCLANS FOR TRANSFER AND EXPRESSION GENE ENCODING ERWINIA CAROTOVORA (ECAR-LANS) L-ASPARAGINASE IN ESCHERICHIA COLI CELLS AND METHOD FOR PREPARING RECOMBINANT ECAR-LANS FROM BIOMASS OF ESCHERICHIA COLI PRODUCER STRAINS | 2002 |
|
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| METHOD FOR PRODUCING A SUBSTANCE OF THE RECOMBINANT L-ASPARAGINASE FROM ERWINIA CAROTOVORA | 2010 |
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RU2300566C2 |
| RECOMBINANT DNA ENCODING FUNCTIONALLY ACTIVE HYBRID PROTEIN OF D-AMINO ACID OXYDASE WITH CHITIN-COUPLING DOMAIN (DAOcbd), pVR1 RECOMBINANT PLASMID PROVIDING SYNTHESIS THEREOF IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C 41 (DE3)/pVR1 AS PRODUCER OF DAOcbd | 2006 |
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| ESCHERICHIA COLI L-ASPARAGINASE PRODUCER AND EXPRESSION PLASMID pET28a-AsnSYN CODING L-ASPARAGINASE | 2023 |
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RU2817891C1 |
| RECOMBINANT DNA, WHICH CODES FUNCTIONALLY ACTIVE HYBRID PROTEIN GL7ACA-ACYLASE WITH CHITIN-BINDING DOMAIN (BrdGL7ACA-cbd) AND RECOMBINANT PLASMID pSVH0108, PROVIDING FOR ITS SYNTHESIS IN CELLS Escherichia coli, RECOMBINANT STRAIN Escherichia coli BL21(DE3)/pSVH0108-PRODUCER BrdGL7ACA-cbd | 2006 |
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RU2388826C2 |
| MODIFIED TSA GENE ENCODING THE THERMOSTABLE L-ASPARAGINASE FROM THERMOCOCCUS SIBIRICUS ARCHAEA, RECOMBINANT ESCHERICHIA COLI STRAIN - THERMOSTABLE L-ASPARAGINASE PRODUCER AND METHOD FOR PURIFICATION OF THIS ENZYME | 2022 |
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