METHOD FOR PREPARING MIXED VACCINE AGAINST COLI-BACTERIOSIS, SALMONELLOSIS AND STREPTOCOCCOSIS IN NUTRIA Russian patent published in 2007 - IPC A61K39/116 A61K39/112 A61K39/108 A61K39/09 

FIELD: biotechnology, microbiology.

SUBSTANCE: invention relates to a method for preparing harmless, specific, highly immune, inactivated, mixed vaccine against coli-bacteriosis, salmonellosis and streptococcosis in nutria wherein method involves using antigens, inactivation and addition of adjuvant. Method involves selection of damaged organs from dead nutrias in local epizootic focus and preparing suspension from these organs followed by inoculation in differential diagnostic media. Pure cultures of coli-bacteriosis, salmonellosis and streptococcosis pathogens are isolated. Cultures Escherichia coli, Salmonella typhimurium and Streptococcus pneumoniae are grown separately in beef-extract broth with addition of 0.2% of glucose and with titer value 4-5 billions microbial cells/1 cm³. Inactivation is carried out by addition of formalin in the final concentration 0.4-0.6%. Then cultures are kept at temperature 37°C for 72-96 h and mixed their in equal ratio. Then aluminum hydroxide solution is added in the amount 20% to volume, stirred thoroughly, packaged and sealed. Method provides preparing harmless, immunogenic and highly specific vaccine.

EFFECT: improved preparing method.

1 tbl, 1 ex