MIXED VACCINE AGAINST COLI-BACTERIOSIS, SALMONELLOSIS AND STREPTOCOCCOSUS IN NUTRIA Russian patent published in 2007 - IPC A61K39/116 A61K39/112 A61K39/108 A61K39/09 A61P1/00 

FIELD: biotechnology.

SUBSTANCE: vaccine comprises as antigens the cell suspension of pure cultures of pathogens of coli-bacteriosis Escherichia coli, salmonellosis Salmonella typhimurium and streptococcosus Streptococcus pneumoniae obtained by selection of damaged organs from dead nutrias and inoculation of cultures on differential-diagnostic media. Isolated pure cultures are grown separately in beef-extract broth with glucose to the concentration of microbial cells 4-5 billions in 1 cm³ followed by their mixing in equal ratio. Vaccine comprises formalin and aluminum hydroxide in the following ratio of components, wt.-%: cell suspension of pure culture of coli-bacteriosis pathogen Escherichia coli isolated from a local epizootic focus in nutrient medium with a titer 4-5 billions of microbial cells in 1 cm³, 24.0-29.0; cell suspension of pure culture of salmonellosis pathogen Salmonella typhimurium isolated from a local epizootic focus in nutrient medium with a titer 4-5 billions of microbial cells in 1 cm³, 24.0-29.0; cell suspension of pure culture of streptococcosus pathogen Streptococcus pneumonia isolated from a local epizootic focus in nutrient medium with a titer 4-5 billions of microbial cells in 1 cm³, 24.0-29.0; glucose, 1.0-2.0; formalin, 1.5-2.0, and aluminum hydroxide, the balance. Proposed vaccine shows high immunogenicity, high specificity and harmless.

EFFECT: valuable properties of vaccine.

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