MIXED VACCINE AGAINST COLI-BACTERIOSIS, SALMONELLOSIS AND ENTROCOCCUS INFECTION IN NUTRIA Russian patent published in 2007 - IPC A61K39/116 A61K39/112 A61K39/108 A61K39/09 A61P1/00 

FIELD: biotechnology, microbiology.

SUBSTANCE: vaccine comprises cell suspension of pure cultures of Escherichia coli as coli-bacteriosis pathogen, Salmonella typhimurium as salmonellosis pathogen and Streptococcus faecalis as enterococcus pathogen as antigens and prepared by selection of damaged organs from dead nutrias from local epizootic focus, preparing suspension, inoculation in differential diagnostic media, isolation of pure cultures of pathogens. Their culturing is carrying out separately in beef-extract broth with glucose up to the concentration 4-5 billions microbial cells/1 cm³ and mixed in equal ratios. Vaccine comprises formalin and aluminum hydroxide in the following ratio of component, wt.-%: cell suspension of pure culture of Escherichia coli as pathogen of coli-bacteriosis isolated from local epizootic focus in nutrient medium with titer value 4-5 billions microbial cells/1 cm³, 24.0-29.0; cell suspension of pure culture of Salmonella typhimurium as pathogen of salmonellosis isolated from local epizootic focus in nutrient medium with titer value 4-5 billions microbial cells/1 cm³, 24.0-29.0; cell suspension of pure culture of Streptococcus faecalis as pathogen of enterococcus infection isolated from local epizootic focus in nutrient medium with titer value^{4-5 billions microbial cells/1 cm3, 24.0-29.0; glucose, 1.0-2.0; formalin, 1.5-2.0, and aluminum hydroxide, the balance. Vaccine shows high immunogenicity, high specificity and safety.}

EFFECT: valuable properties of vaccine.

1 tbl, 5 ex