METHOD FOR PREPARING ASSOCIATED VACCINE AGINST SALMONELLOSIS AND COLIBACTERIOSIS IN NUTRIAS Russian patent published in 2007 - IPC A61K39/116 A61K39/112 A61K39/108 

FIELD: veterinary medicine.

SUBSTANCE: for manufacturing an associated vaccine it is necessary to select affected organs in dead nutrias from local epizootic focus for the purpose to prepare the suspension and fulfill inoculation onto differentially-diagnostic media. One should isolate pure cultures and separately grow the cultures of salmonellosis agents Salmonella typhimurium and Escherichia coli in beef-extract broth at addition of 0.2%-glucose at the titer being about 4-5 bln. microbial cells/cu. cm followed by inactivation due to applying formalin up to 0.4-0.6%-final concentration to be kept at 37°C for about 72-96 h. Cultures should be mixed at equal ratios, then aluminum hydroxide solution should be applied at the quantity of 20% against the culture volume to be then thoroughly mixed, packed and sealed. The innovation enables to obtain safe, specific and high-immunogenic vaccine.

EFFECT: higher efficiency.

1 ex, 1 tbl