METHOD FOR MANUFACTURING ASSOCIATED VACCIONE AGAINST COLIBACTERIOSIS, SALMONELLOSIS, STREPTOCOCCOSIS AND ENTEROCOCCAL INFECTION IN NUTRIAS Russian patent published in 2008 - IPC A61K39/116 A61K39/09 A61K39/108 A61K39/112 

FIELD: veterinary medicine.

SUBSTANCE: it is necessary to select the affected organs from dead nutrias out of local epizootic focus, prepare the suspension out of pathological material to inoculate onto differential-diagnostic media, isolate pure cultures of Escherichia coli, Salmonella typhimurium, Streptococcus pneumoniae and Streptococcus fecalis, grow separately the isolated cultured in beef-extract nutritive medium at addition of 0.2%-glucose to achieve the concentration of microbial cells of about 4-5 bln/cu/ cm, inactivate with formalin up to 0.4-0.6%-final concentration, keep at 37°C for about 72-96 h, mix the cultures at equal ratios, add 3%-aluminum hydroxide solution at the quantity of 20% against the volume of the culture to be thoroughly mixed. Then the vaccine obtained should be packed and sealed. The innovation is simple in usage, moreover, it enables to increase specificity and immunogenicity of the obtained vaccine.

EFFECT: higher efficiency.

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