FIELD: veterinary medicine.
SUBSTANCE: method involves taking 10-100 samples of about 1 g large weight. They are thoroughly mixed with water and precipitated in single laboratory glass, filtering twice, washing the filtered suspension 3-5 times, centrifuging the precipitate 1-2 times at 1500 rpm, pouring the supernatant out and adding combined floatation fluid to the precipitate. Saturated ZnCl2 (2 kg per 1 l), NaCl (0.42 kg per 1 l) and sugar (1.67 kg per 1 l) solutions taken in 2:1:1 proportion are used as the floatation fluid. After having added floatation fluid, the floatation fluid precipitate is shaken up to produce suspension and centrifuged repeatedly in the same mode. The supernatant liquid is poured out and the floatation fluid is added to obtain convex meniscus. It is covered with object-plate for 3-5 min. Then, the liquid is studied with microscope on glass.
EFFECT: accelerated diagnosis method.
1 tbl
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Authors
Dates
2007-07-10—Published
2004-04-19—Filed