FIELD: medicine.
SUBSTANCE: method involves applying high-performance liquid chromatography in supernatant produced by precipitating blood plasma proteins with acetonitrile. To do it, Pronto SIL-120-5-C18AQ chromatographic column of 2x75 mm with particle size of 5 mcm is used. 0.1 M sodium dihydrophosphate solution and acetonitrile mixture is used as eluent with acetonitrile concentration gradient being from 0 to 50% and eluent flow supply rate being equal to 90 mcl/min, eluting volume 1500 mcl, chromatographic column temperature 35°C. Detection is carried out at wavelength of 210 nm.
EFFECT: high accuracy in determining histamine concentration.
2 dwg, 1 tbl
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