FIELD: medicine.
SUBSTANCE: invention refers to medicine, namely pharmacology and toxicology, and can be used to determine N-[3-(4-nitrophenylamino)-indol-2-ylmethylene]aminoguanidine methanesulphonate in blood plasma. For this purpose, the sample is extracted with acetonitrile with addition of sodium chloride. Then, the preparation is quantitatively determined by high-performance liquid chromatography on a ProntoSIL column, 75x2.0 mm, 5 mcm in a gradient elution mode, 0.2 M lithium perchlorate solution in 0.005 M chloric acid solution is used as the mobile phase A of the mixture, acetonitrile is used as mobile phase B with subsequent UV detection at wavelength of 330 nm.
EFFECT: invention provides quantitative determination of N-[3-(4-nitrophenylamino)-indol-2-ylmethylene]aminoguanidine methanesulphonate in biological media.
1 cl, 1 dwg, 6 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR THE QUANTITATIVE DETERMINATION OF 2-ISOPROPYL-5-METHYLCYCLOHEXYL 2-(1-(4-CHLOROBENZOYL)-5-METHOXY-2-METHYL-1H-INDOL-3-YL)ACETATE AND INDOMETHACIN IN BLOOD PLASMA | 2021 |
|
RU2781342C1 |
| METHOD FOR QUANTITATIVE DETERMINATION OF 4-AMINO-1-(3-NITRO-2-OXO-1-PHENYL-1,2-DIHYDRO-1,6-NAPHTHYRIDIN-5-YL) PYRIDINIUM CHLORIDE IN BIOLOGICAL MEDIA | 2018 |
|
RU2686116C1 |
| NEW BIOLOGICALLY ACTIVE COMPOUND N-[3-(4-NITROPHENYLAMINO)-INDOL-2-YLMETHYLENE]AMINOGUANIDINE HYDROCHLORIDE WITH ANTIINFLAMMATORY ACTIVITY | 2010 |
|
RU2478618C2 |
| METHOD OF QUANTITATIVE DETERMINATION OF 8-(TRIFLUOROMETHYL)BENZO[f][1,2,3,4,5]PENTATHIEPIN-6-AMINE HYDROCHLORIDE IN BIOLOGICAL MEDIA | 2018 |
|
RU2676487C1 |
| METHOD FOR QUANTITATIVE DETERMINATION OF DISULPHIRAM IN BIOLOGICAL MEDIA | 2019 |
|
RU2701524C1 |
| METHOD FOR QUANTITATIVE DETERMINATION OF 2-PHENOXYETHANOL IN BIOLOGICAL MEDIA | 2021 |
|
RU2776730C1 |
| METHOD OF QUANTITATION 2,2,6,6-TETRAMETHYL-N-{1-[5-(4-METHYL-3-CHLOROANILINE)-1,2,4-THIADIAZOLE-3-YL]PROPAN-2-YL}PIPERIDIN-4-AMINE DIHYDROCHLORIDE IN BIOLOGICAL MATRICES | 2016 |
|
RU2636231C1 |
| METHOD FOR QUANTITATIVE DETERMINATION OF DEXAMETHASONE IN BIOLOGICAL MEDIA USING HPLC WITH ULTRAVIOLET DETECTION | 2022 |
|
RU2792274C1 |
| METHOD FOR HPLC-BASED DETERMINATION OF VERAPAMIL ENANTIOMERS IN ACTIVE PHARMACEUTICAL INGREDIENTS, TABLETS AND BLOOD PLASMA SAMPLES | 2008 |
|
RU2395807C1 |
| METHOD FOR QUANTITATIVE DETERMINATION OF DOXORUBICIN IN BIOLOGICAL SAMPLES | 2024 |
|
RU2839330C1 |
