FIELD: biotechnology, in particular modification of galactomannanes in green coffee beans by decreasing of endogen levels of α-D-galactosidase activity.
SUBSTANCE: claimed method includes preparation of coffee plant cell containing nucleic acid, transcribed to ribonucleic acid which antisense one in relates to mRNA derived from α-D-galactosidase gene under control of constitutive or inducible promoter. Further method includes production of plant containing such cell; instant coffee manufacturing from bean of said plant. Coffee solubility in increased by using of bean of said plant.
EFFECT: prevention of raw green coffee mass losses; instant coffee drinks of prolonged storage time without losses of quality.
5 cl
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