ARTIFICIAL INSEMINATION OF MAMMALIANS WITH LOW QUANTITY OF SEX-SEPARATED SPERMATOZOA Russian patent published in 2007 - IPC A01K67/02 

FIELD: animal science.

SUBSTANCE: for the purpose to obtain farm animals of preset sex it is necessary to apply male spermatozoa to detect sex sign in certain multitude of spermatozoa and sort spermatozoa according to the detected sex sign and collect them into the collector supplied with a shock absorbing element. Then one should obtain a sorted combination of spermatozoa to introduce it for female of the given mammalian type to fertilize, at least, one female ovicell at resultativeness level being, at least, 50% against that of pregnancy achieved at applying conventional combination of spermatozoa for artificial insemination. For separating spermatozoa one should create the source of cells that supplies spermatozoa for sorting procedure. Chemically one should coordinate capsular liquid for creating the medium out of capsular liquid for spermatozoa being coordinated with liquid medium of spermatozoa both before and after sorting technique. Then it is important to identify one of spermatozoa's properties. Spermatozoa should be differentiated in accordance to their sex and separated at the rate of, at least, 1200 operations/sec. Then spermatozoa with desired sex sign should be collected into the collector supplied with shock-absorbing element. To obtain multiple embryos it is necessary to induce polyovulation in a female of the given mammalian type to obtain, at least, two ovicells. Moreover, a polyovulation-favoring pharmaceutical preparation should be introduced at 12-h-long interval at any days between the 2^nd and the 18^th d of estrus cycle. It is necessary to detect sex in certain multitude of spermatozoa in a male of the given mammalian type to separate these spermatozoa according to sex sign and introduce separated spermatozoa into female uterus of the given mammalian type after estrus onset to fertilize multitude of ovicells in the uterus. The innovation enables to efficiently separate male spermatozoa in farm animals and obtain from them the offspring of desired sex and, also, obtain multiple embryos of the preset sex.

EFFECT: higher efficiency of artificial insemination.

70 cl, 4 dwg, 4 ex