FIELD: molecular biology, medicine.
SUBSTANCE: disclosed is improved method for simultaneous genetic typing of H63D and C282Y polymorphisms in HFE gene by using PCR method in real time with fluorescent detection. Claimed method includes a) step of reaction mixture containing no analyzed DNA preincubation at +4°C for at least 1 hour; b) application of "hot start" method to activate reaction and c) selection of optimal pairs of fluorescent dye/quenching agent, namely FAM and BHQ1 for normal variant 63H probe; R6G and BHQ1 for mutant variant 63D probe; ROX and BHQ2 for normal variant 282C probe; and Cy5 and BHQ3 for mutant variant 282Y probe. Claimed method is fully adapted to clinical application.
EFFECT: method with high specificity level.
10 dwg, 2 tbl 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF IDENTIFYING S65C, H63D, C282Y MUTATIONS AND POLYMORPHISM 2 EX + 4T>C IN HFE GENE | 2008 |
|
RU2396354C2 |
| METHOD FOR DETECTING GENETIC PREDISPOSITION TO RECURRENT PREGNANCY LOSS | 2012 |
|
RU2532367C2 |
| APPLICATION OF DNA-DIAGNOSTICS FOR PRESENCE OF POLYMORPHISM R353Q IN GENE OF FACTOR VII OF BLOOD COAGULATION FOR ESTIMATION OF PREDISPOSITION TO DEVELOPMENT OF HABITUAL NONCARRYING OF PREGNANCY AND METHOD OF PREDISPOSITION ESTIMATION TO THIS DISEASE BY MEANS OF ANALYSIS OF COMBINATION OF POLYMORPHISMS C677T OF GENE MTHFR AND OF GENE R353Q OF FACTOR VII | 2006 |
|
RU2330071C1 |
| METHOD OF DETECTION OF DELETION IN DAT RAT LINE | 2023 |
|
RU2811140C1 |
| METHOD FOR POINT MUTATION DIAGNOSING IN NATIVE DNA USING GRAPHENE OXIDE | 2015 |
|
RU2614111C1 |
| METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT rs13056243 (C>T) OF HUMAN ZNRF3 GENE BY REAL-TIME POLYMERASE CHAIN REACTION | 2023 |
|
RU2819836C1 |
| METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT OF rs5762821 (G>A) OF HUMAN LONG NON-CODING RNA GENE (LNCRNA, ENSEMBL ID: ENSG00000226471) BY REAL-TIME POLYMERASE CHAIN REACTION | 2024 |
|
RU2825465C1 |
| METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT rs12169782 (G>A) OF HUMAN TTC28-AS1 GENE BY REAL-TIME POLYMERASE CHAIN REACTION | 2024 |
|
RU2826721C1 |
| METHOD FOR GENOTYPING SINGLE-NUCLEOTIDE VARIANT rs71327329 (A>G) OF THE HUMAN ZNRF3 GENE BY REAL-TIME POLYMERASE CHAIN REACTION | 2023 |
|
RU2821589C1 |
| METHOD OF DIAGNOSING MUCOPOLYSACCHARIDOSIS-PLUS SYNDROME | 2022 |
|
RU2798695C1 |
