FIELD: chemistry of polymers, biochemistry.
SUBSTANCE: invention relates to a rapid method for preparing photoreactive polymers and immobilization of biomolecules on indicated polymers. Invention describes a method for preparing photoreactive polymers followed by immobilization of molecules on them that involves the following steps: (a) effect of microwave radiation on mixture of photolinker and solvent; (b) washing out polymer with a corresponding solvent followed by drying at the environment temperature to obtain photoreactive polymer; (c) addition of biomolecule dissolved in corresponding buffer on photoreactive polymer; (d) treatment of mixture by the photoenergy source for period from 2 min to 2 h for immobilization of molecule on indicated polymer, and (e) washing out indicated polymer having immobilized molecule in corresponding buffer and the following drying indicated polymer at the environment temperature to obtain photoreactive polymer with molecules immobilized on its.
EFFECT: improved preparing method.
22 cl, 2 tbl, 18 ex
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Authors
Dates
2007-10-27—Published
2003-03-31—Filed