METHOD FOR PREPARING PHOTOREACTIVE POLYMERS FOR IMMOBILIZATION BIOMOLECULES ON THEM Russian patent published in 2007 - IPC C12N11/08 G01N33/543 

Abstract RU 2309180 C2

FIELD: chemistry of polymers, biochemistry.

SUBSTANCE: invention relates to a rapid method for preparing photoreactive polymers and immobilization of biomolecules on indicated polymers. Invention describes a method for preparing photoreactive polymers followed by immobilization of molecules on them that involves the following steps: (a) effect of microwave radiation on mixture of photolinker and solvent; (b) washing out polymer with a corresponding solvent followed by drying at the environment temperature to obtain photoreactive polymer; (c) addition of biomolecule dissolved in corresponding buffer on photoreactive polymer; (d) treatment of mixture by the photoenergy source for period from 2 min to 2 h for immobilization of molecule on indicated polymer, and (e) washing out indicated polymer having immobilized molecule in corresponding buffer and the following drying indicated polymer at the environment temperature to obtain photoreactive polymer with molecules immobilized on its.

EFFECT: improved preparing method.

22 cl, 2 tbl, 18 ex

Similar patents RU2309180C2

Title Year Author Number
QUICK, HEATING-MEDIATED METHOD FOR FULFILLING SORPTION IMMUNOENZYMATIC ASSAY 2003
  • Nakhar Pradip
  • Bora Utpal
RU2309407C2
IMMOBILIZED PROTEIN MATERIAL AND USE THEREOF AS HETEROGENOUS CATALYSTS 2015
  • Kassime Karim Engelmark
  • Bekvall Yan-Erling
RU2684619C2
QUICK METHOD FOR CARRYING OUT MICROWAVE-MEDIATED SOLID-STATE IMMUNOENZYME ANALYSIS 2000
  • Nakhar Pradeep
  • Bora Utpal
  • Sharma Gainda Lal
RU2249218C2
CONJUGATE OF NANODIAMOND WITH PYROPHOSPHATASE AND METHOD OF OBTAINING THEREOF 2013
  • Jakovlev Ruslan Jur'Evich
  • Rodina Elena Valer'Evna
  • Valueva Anastasija Viktorovna
  • Vorob'Eva Natalija Nikolaevna
  • Lisichkin Georgij Vasil'Evich
  • Leonidov Nikolaj Borisovich
RU2542411C1
METHOD FOR TRAY SENSITISATION FOR POLYSACCHARIDE ANTIGEN ENZYME IMMUNOASSAY 2008
  • Belik Timur Viktorovich
RU2380709C1
METHOD OF PRODUCING SURFACE-MODIFIED NANOPARTICLES FOR IMMOBILISING BIOLOGICAL SUBSTANCES 2010
  • Prosekov Aleksandr Jur'Evich
  • Soldatova Ljubov' Sergeevna
  • Babich Ol'Ga Olegovna
RU2425879C1
METHOD FOR PRODUCING A HETEROGENEOUS BROMELINE PREPARATION WHICH IS COVALENTLY BOUND TO A CHITOSAN MATRIX 2018
  • Kholyavka Marina Gennadevna
  • Olshannikova Svetlana Sergeevna
  • Artyukhov Valerij Grigorevich
RU2711786C1
METHOD OF PRODUCING ALPHA-FETOPROTEIN IMMOBILIZED ON PET MICROPARTICLES 2018
  • Kuznetsova Viktoriya Evgenevna
  • Miftakhov Rinat Azatovich
  • Lapa Sergej Anatolevich
  • Spitsyn Maksim Anatolevich
  • Shershov Valerij Evgenevich
  • Gusejnov Tejmur Oktaevich
  • Timofeev Eduard Nikolaevich
  • Radko Sergej Pavlovich
  • Lisitsa Andrej Valerevich
  • Chudinov Aleksandr Vasilevich
RU2731415C2
PHOTOBIOCATALYST FOR PRODUCING REDUCED FORMS OF NICOTINAMIDE COENZYMES NADH OR NADPH AND PHOTOCATALYTIC METHOD FOR PRODUCING NADH OR NADPH 2009
  • Nikandrov Vitalij Viktorovich
  • Nadtochenko Viktor Andreevich
  • Semenov Aleksej Jur'Evich
  • Borisova Janina Vladimirovna
  • Nizova Galina Viktorovna
  • Pulinets Inna Anatol'Evna
  • Alisova Irina Vladimirovna
  • Kostrov Andrej Nikolaevich
  • Sarkisov Oleg Mikhajlovich
RU2416644C1
METHOD OF SYNTHESIS OF FUNCTIONAL CARBON QUANTUM DOTS 2023
  • Korenkov Egor Sergeevich
  • Nikitin Maksim Petrovich
RU2824005C1

RU 2 309 180 C2

Authors

Nakhar Pradip

Nakvi Azmi

Dates

2007-10-27Published

2003-03-31Filed