FIELD: biotechnology, preparative biochemistry, biopharmacology, enzymes.
SUBSTANCE: invention relates to a method for preparing recombinant L-asparaginase protein EcA2. Method involves constructing plasmid pNAN5 determining synthesis of L-asparaginase EcA2 and transformation of Bacillus cereus 1676 cells with this plasmid. Strain-producer is cultured in nutrient medium and bacterial mass is removed by filtration. Cultural fluid is concentrated in hollow fibers followed by gel-filtration in ultragel Sephacryl S200 and concentrating in an ultrafiltering cell. Invention provides enhancing effectiveness of synthesis of L-asparaginase EcA2.
EFFECT: improved preparing method, valuable properties of plasmid and strain.
4 cl, 4 dwg, 3 ex
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