RECOMBINANT PLASMID DNA pL-ASP-08 AND Escherichia coli XL1-blue/pL-ASP-08 BACTERIA STRAIN - L-ASPARAGINASE PRODUCER Russian patent published in 2010 - IPC C12N15/00 C12N1/21

Abstract RU 2397248 C1

FIELD: chemistry; biochemistry.

SUBSTANCE: invention relates to biotechnology, particularly to genetic engineering and pertains to a novel plasmid pL-ASP-08 and a novel Escherichia coli XL 1-blue/pL-ASP-08 bacteria strain containing the said plasmid. The recombinant plasmid DNA pL-ASP-08 has molecular weight of 2.9 MDa (4471 base pairs) and is designed for L-asparaginase synthesis.

EFFECT: invention enables to obtain highly pure genetically engineered L-asparaginase for preparing medicinal agents used in treating lymphoblastic leucosis, lympho- and reticulosarcoma in combined chemotherapy.

2 cl, 1 dwg, 2 ex

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RU 2 397 248 C1

Authors

Shibanova Elena Dmitrievna

Kosarev Sergej Anatol'Evich

Uznadze Ol'Ga Leonidovna

Dates

2010-08-20—Published

2009-05-07—Filed