METHOD FOR PREPARING PURIFIED CHOLERA TOXIN B SUBUNIT FROM RECOMBINANT STRAIN VIBRIO CHOLERAE Russian patent published in 2012 - IPC A61K39/00 C07K14/28 C12P21/00 C12R1/63 

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention discloses a method for preparing a major protective antigen of a cholera vibrio of cholera toxin B subunit. The method involves deposition of a protein fraction from filtered broth culture of the recombinant strain other than 01 serogroup Vibrio cholerae KM93 (State Collection 'Microbe'). The deposited protein fraction is used to recover the B subunit to be purified by three-step column TSK HW-60 gel penetration chromatography. The purified preparation of the B subunit is applicable for producing anti-toxin serums, high-specific immunoglobulin and diagnostic test systems, as well as an ingredient of vaccine preparations.

EFFECT: use of the invention enables higher yield of the high-purity native immunogenic preparation of the cholera toxin B subunit.

2 dwg, 1 tbl