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2 701 145

(13)

(51)

IPC

A61K39/125(2006-01-01)

(21) (22)

Application

2019121521, 2019-07-10

(24)

Start date

2019-07-10

(22)

Actual filing date

2019-07-10

(45)

Published

2019-09-25

(72)

Inventor

Rezajkin Aleksej VasilevichSharabrin Sergej ValerevichUsoltseva Polina SergeevnaAlimov Aleksandr Viktorovich

(73)

Holder

Federalnoe Byudzhetnoe Uchrezhdenie Nauki Nauchno-Issledovatelskij Institut Virusnykh Infektsij" Federalnoj Sluzhby Po Nadzoru V Sfere Zashchity Prav Potrebitelej I Blagopoluchiya Cheloveka Rospotrebnadzora)

METHOD FOR ENTEROVIRUS GENOTYPING BY GENOME REGION 1A-1B SEQUENCING METHOD Russian patent published in 2019 - IPC A61K39/125

Abstract RU 2701145 C1

FIELD: biology.

SUBSTANCE: invention relates to molecular biology, laboratory diagnostics, virology and epidemiology and aims at genotyping enterovirus by sequencing 1A-1B genome. Viral RNA is recovered, cDNA is obtained, which is used as a matrix in the reaction of "half-embedded" polymerase chain reaction. Genotyping of detected enteroviruses is performed by a method of comparative analysis of sequences obtained after amplicon sequencing, with sequences of genomes of enteroviruses from an international database of genetic data.

EFFECT: invention provides developing a more sensitive method of enterovirus genotyping.

1 cl

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RU 2 701 145 C1

Authors

Rezajkin Aleksej Vasilevich

Sharabrin Sergej Valerevich

Usoltseva Polina Sergeevna

Alimov Aleksandr Viktorovich

Dates

2019-09-25—Published

2019-07-10—Filed