FIELD: biotechnology.
SUBSTANCE: invention is related to cryopreservation of animal cells and can be used in medicine. A method of cryopreservation has been proposed, including: cooling cells to a temperature from 0°С to +6°C, introduction cryoprotectors into them by diffusion and exposure at a given temperature. During the process of diffusion, animal cells are saturated with heavy water to a concentration of 5 to 20 vol.% of heavy water from the total amount of water in the cells. After exposure, the cells are cooled to a temperature of -7°С to -195.75°C and stored at this temperature.
EFFECT: advantage of the proposed cryopreservation method is a reduction in cryodamage and an increase in cell survival.
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