METHOD FOR PREPARING MEDICINAL AGENT FOR TREATMENT OF MYOPIA AND EYE SCLERAL ENVELOPE DISEASES AND VITREORETONOPATHY Russian patent published in 2008 - IPC A61K35/44 A61K38/17 A61P27/02 

FIELD: medicine, ophthalmology, preparative biochemistry.

SUBSTANCE: method involves isolation of tissue of scleral envelope central region from fresh enucleated eye of vertebrate animals wherein this tissue comprises a clibriform plate. Clibriform plate is washed out, kept in aqueous-saline physiological solution, centrifuged and supernatant is collected. Supernatant is separated by its dissolving in 100% solution of ammonium sulfate, filtered and dialyzed up to the complete removal of ammonium sulfate ions followed by separation by isoelectrofocusing method in sucrose gradient density at pH 3.5-10.0, at temperature 4-6°C and voltage 500-2000 V for 72-96 h. Then fractions of acid proteins are collected, combined and dialyzed up to the complete removal of sucrose and ampholines. Prepared protein aqueous solution is dried up to preparing a dry matter that is purified by electrophoresis method in 7.5-15% polyacrylamide gel and low-molecular fraction with value R_f = 0.9-0.95 containing a regulatory peptide is collected by elution from gel with deionized water at temperature 4-7^oC for 2-5 days. Prepared fraction is dialyzed in dialysis sacs with the penetration limit value of pores 2-8 kDa at temperature 4-7^oC for 7-10 days with deionized water. After dialysis the regulatory peptide solution is dried again up to preparing flaky-like finely dispersed powder that is dissolved then in physiological aqueous-saline solution. Invention provides the enhanced percent yield of regulatory peptides possessing biological activity in superlow doses and isolation of highly purified polypeptides of low-molecular mass.

EFFECT: improved preparing method.

6 ex