METHOD FOR PREPARING MEDICINAL AGENT FOR TREATMENT OF EYE RETINA AND PIGMENT EPITHELIUM AND EYE VASCULAR ENVELOPE Russian patent published in 2007 - IPC A61K35/44 A61K38/17 A61P27/02 

FIELD: medicine, ophthalmology.

SUBSTANCE: method involves isolation of macular region of retina pigment epithelium from freshly enucleated eye of vertebrate animals, its washing out in physiological solution, holding pigment epithelium tissue in aqueous-saline solution at 4-7⁰C for 2-4 h followed by centrifugation. Supernatant liquid is collected, its components are separated by isoelectrofocusing method in sucrose gradient density at pH 3.5-10.0. Fractions of basic proteins are collected and sucrose and ampholines are removed by dialysis. Prepared protein solution is dried and purified by electrophoresis on 7.5-15% polyacrylamide gel. Then low-molecular fraction with value Rf = 0.4-0.9 is eluted with deionized water in dialysis sacs with penetration limit of pores 2-8 kDa, and prepared regulatory peptide is dried and dissolved in physiological aqueous-saline solution. Invention provides enhancing percent yield of regulatory peptides possessing the biological activity in superlow doses, and isolation of highly purified polypeptides of low molecular mass.

EFFECT: improved preparing method of medicinal agent.

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