METHOD FOR PREPARING MEDICINAL AGENT FOR CATARACT TREATMENT Russian patent published in 2008 - IPC A61K35/44 A61K38/17 A61P27/02 

FIELD: medicine, ophthalmology, pharmacy, preparative biochemistry.

SUBSTANCE: method involves isolation of undamaged lens from fresh enucleated eyes of vertebrate animals. Lenses are kept in an aqueous-saline solution, tissue extract is centrifuged and supernatant is collected. Supernatant is separated by its dissolving in 100% ammonium sulfate solution, dialyzed up to the complete removal of ammonium sulfate ions and separated by isoelectrofocusing method in sucrose gradient density at pH 3.5-10.0. Fractions of acid proteins are collected, dialyzed up to the complete removing sucrose and ampholines and prepared protein aqueous solution is dried and purified by electrophoresis in 7.5-15% polyacrylamide gel. Low-molecular fraction with a value R_f = 0.9-0.95 is eluted from gel with deionized water at temperature 4-7°C for 2-5 days, and prepared fraction is dialyzed in dialysis sacs with penetration limit value of pores 2-8 kDa at temperature 4-7°C for 7-10 days. Prepared regulatory peptide is dried and dissolved in physiological aqueous-saline solution. Invention provides the enhanced percent yield of regulatory peptides possessing biological activity in superlow doses and isolation of highly purified polypeptides of low molecular mass.

EFFECT: improved preparing method.

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