METHOD FOR DETERMINATION OF HUMAN 8-OXOGUANINE-DNA-GLYCOSILASE ACTIVITY Russian patent published in 2008 - IPC C12Q1/25 

Abstract RU 2321637 C1

FIELD: biochemistry.

SUBSTANCE: method involves mixing solutions of enzyme and DNA-substrate containing residue of 8-oxoguanine taken in the concentration 0.5-4 mcM in the ratio 1:1. Activity of enzyme is determined by recording change of intensity of its fluorescence in reaction medium in the time interval 1-1000 s. Method provides enhancing precision and reliability of assay activity of enzyme.

EFFECT: improved method of determination.

3 cl, 2 dwg, 1 ex

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RU 2 321 637 C1

Authors

Fedorova Ol'Ga Semenovna

Koval' Vladimir Vasil'Evich

Kuznetsov Nikita Aleksandrovich

Dates

2008-04-10Published

2006-06-07Filed