FIELD: biochemistry.
SUBSTANCE: method involves mixing solutions of enzyme and DNA-substrate containing residue of 8-oxoguanine taken in the concentration 0.5-4 mcM in the ratio 1:1. Activity of enzyme is determined by recording change of intensity of its fluorescence in reaction medium in the time interval 1-1000 s. Method provides enhancing precision and reliability of assay activity of enzyme.
EFFECT: improved method of determination.
3 cl, 2 dwg, 1 ex
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Authors
Dates
2008-04-10—Published
2006-06-07—Filed