FIELD: biotechnology.
SUBSTANCE: disclosed is a recombinant bacterial strain Escherichia coli which is a producer of chimeric protein formamidopyrimidine-DNA-glycosylase (FPG) with a fluorescent reporter protein (EGFP). Said strain is obtained by transformation of cells Escherichia coli strain BL21 (DE3) plasmid pET24b(+)-Fpg-(G4S)3-TEVs-EGFP, having a nucleotide sequence SEQ ID NO: 1, constructed on the basis of the pET24b (+) vector and containing the f1 replication origin, the T7 phage promoter, the RBS ribosome entry site, built-in gene coding chimeric recombinant protein FPG and EGFP, having amino acid sequence SEQ ID NO: 2, a phage T7 terminator, a kanamycin resistance gene Kan (R), a lactose repressor lacI and a lactose operator.
EFFECT: invention enables to obtain a recombinant producer strain which enables to obtain a chimeric recombinant protein formamidopyrimidine-DNA glycosylase (Fpp) with a fluorescent reporter protein (EGFP) capable of forming a DNA-protein cross-link.
1 cl, 8 dwg, 4 ex
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