FIELD: medicine, proteins, preparative biochemistry.
SUBSTANCE: invention relates to method for preparative methods for preparing collagen. Method involves preparing collagen from biological material by its mechanical milling, treatment with alkaline solution, washing out with water, dissolving in acetic acid followed by isolation of the end product. Method involves cutting the biological material for pieces of size 0.5-1 cm2, and their treatment is carried out with 10-20% alkaline solution for 1 h, and collagen dissolved in acetic acid is added to acetone at the constant stirring. Invention provides decreasing time for preparing collagen, and economy of reagents.
EFFECT: improved preparing method.
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