FIELD: medicine; laboratory techniques.
SUBSTANCE: to evaluate the stability factor of erythrocytes in hypoxia, patient's blood sample is taken; heparin 0.1 ml is used as a coagulant. The sample is centrifuged at 4000 rpm for 10 min, and the erythrocyte suspension is received. The real part of dielectric constant of erythrocyte suspension is measured immediately after it is obtained and in 60 min, at fixed frequencies - 1 and The polarisation factors (PF1 and PF2) are calculated by formula: , then the stability factor of erythrocytes in hypoxia is calculated (SF): SF=SF2: SF1, and if it is 1.0 and less, the affected erythrocytes stability to hypoxia is diagnosed.
EFFECT: method allows the dynamic evaluation of treatment efficiency in diseases, where the free-radical processes play the key role in pathogenesis.
4 tbl, 3 ex
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Authors
Dates
2008-05-10—Published
2007-02-05—Filed