METHOD FOR PRODUCING LIPIDES CONTAINING POLYUNSATURATED FATTY ACIDS (VARIANTS) AND METHOD FOR CULTIVATING MICROORGANISMS PRODUCING SAID LIPIDS Russian patent published in 2008 - IPC C12P7/64 C12N1/00 C12R1/00 

FIELD: biotechnologies.

SUBSTANCE: the methods provide for cultivating microorganisms of the Thraustochytriales subclass, capable of producing polyunsaturated fatty acids of at least 15% of the total quantity of lipids. Said microorganisms are cultivated in a medium containing an alcohol-free carbon source and a source of limiting nutrients. The alcohol-free carbon source and the source of limiting nutrients are added until a biomass density of at least 100 g/l on dry cells mass basis is achieved, and then the process is carried while providing conditions, under which the limiting nutrient is limited. The concentration of dissolved oxygen is maintained thereby at a lever not exceeding 3% of the saturation.

EFFECT: producing lipids with high concentration of polyunsaturated fatty acids.

65 cl, 1 dwg, 9 tbl, 9 ex