CONTINUOUS METHOD OF CULTURING ALGAE Russian patent published in 2009 - IPC C12N1/12 

FIELD: chemistry; biochemistry.

SUBSTANCE: proposed method involves continuous culturing of an auxotrophic marine microorganism in a fermenter in aerobic conditions, in a culture medium with Y (output), g/l of dry cell substance, CDM. Y equals 100-300 g/l. The culture medium contains a carbon and nitrogen source, added gradually. The carbon source is used in quantity (Y x h) g/l of cultural broth, where h equals 1.1-3.0. The carbon source limits formation of biomass. The nitrogen source is used in quantity (Y x h x f) g/l of culture broth, where f equals 0.002-0.2. The time of stay in the fermenter is 20-100 hours. At least 40% of the obtained biomass consists of components, capable of being extracted using a chloroform and methanol mixture. Output of polyene acids is 0.2 g DHA/l/hr.

EFFECT: high output of polyene acids.

13 cl, 5 ex