FIELD: biotechnology, preparative biochemistry.
SUBSTANCE: invention proposes a method for preparing the recombinant human tumor necrosis factor-aplha (TNF-alpha). Method involves culturing the strain-producer E. coli SG20050/pTNF311Δ, disruption of cells by ultrasonic oscillation, extraction of the end protein and 3-step chromatography purification procedure on DEAE-cellulose column in the linear gradient concentrations of NaCl at pH 8.0, on hydroxyapatite in the linear gradient concentrations of potassium phosphate at pH 8.0 and on hydroxyapatite in the linear gradient concentrations of potassium phosphate at pH 6.7. The recombinant TNF-alpha prepared by the proposed method shows the reduced content of impurity proteins, nucleic acids and lipopolysaccharides especially that provides its direct medicinal using. Invention can be used in medico-biological industry.
EFFECT: improved preparing method, enhanced quality of polypeptide.
2 tbl, 5 dwg, 2 ex
| Title |
Year |
Author |
Number |
| METHOD OF HUMAN RECOMBINANT TUMOR NECROSIS ALPHA-FACTOR PREPARING |
1997 |
- Pustoshilova N.M.
- Kileva E.V.
- Denisova L.Ja.
- Shingareva N.V.
- Korobko V.G.
- Denisov L.A.
- Masycheva V.I.
- Sandakhchiev L.S.
- Kalinin Ju.T.
|
RU2144958C1 |
| METHOD OF PREPARING HUMAN RECOMBINANT TUMOR NECROSIS BETA-FACTOR |
1997 |
- Sinichkina S.A.
- Pustoshilova N.M.
- Masycheva V.I.
- Lebedev L.R.
- Korobko V.G.
|
RU2132385C1 |
| METHOD OF PREPARING POLYPEPTIDE SHOWING HUMAN LYMPHOTOXIN PROPERTY |
1992 |
- Pustoshilova N.M.
- Lebedev L.R.
- Gileva I.P.
- Korobko V.G.
- Davydov I.V.
- Petrenko V.A.
|
RU2048521C1 |
| METHOD OF ISOLATION OF RECOMBINANT HUMAN TUMOR NECROSIS ALPHA-FACTOR |
1992 |
- Bumjalis Vladas Vlado[Lt]
- Ehzerskajte Ina Kosto[Lt]
- Korobko Vjacheslav Grigor'Evich[Ru]
- Janulajtis Ehugenijus-Arvidas Andrjaus[Lt]
|
RU2101292C1 |
| METHOD OF PRODUCTION AND PREPARATION OF ANALOGUE OF HUMAN RECOMBINANT INTERFERON GAMMA |
2006 |
- Miroshnikov Pavel Nikolaevich
- Lebedev Leonid Rudol'Fovich
- Masycheva Valentina Ivanovna
|
RU2326944C2 |
| RECOMBINANT PLASMID DNA pFastBac-G2R-IgG, WHICH CONTAINS FRAGMENT OF SMALLPOX VIRUS GENOME, CODING FACTOR OF TUMOUR NECROSIS, BINDING PROTEIN, AND FRAGMENT OF HUMAN GENOME, CODING SECTION OF HEAVY CHAIN OF ANTIBODY G, AND STRAIN OF BACULOVIRUS BvG2RIgG, WHICH PRODUCES SOLUBLE CHIMERIC PROTEIN, WHICH CONSISTS OF SMALLPOX VIRUS PROTEIN, BINDING FACTOR OF TUMOUR NECROSIS, AND FRAGMENT OF HEAVY CHAIN OF HUMAN ANTIBODY G |
2008 |
- Nepomnjashchikh Tat'Jana Sergeevna
- Gileva Irina Pavlovna
- Lebedev Leonid Rudol'Fovich
- Shchelkunov Sergej Nikolaevich
|
RU2376375C2 |
| RECOMBINANT PLASMID DNA pQE-60-TNFR-CrmB-Ind-67 AND pFastBac1-G2R-dSECRET CONTAINING SMALLPOX VIRUS GENOME FRAGMENT CODING TUMOUR NECROSIS FACTOR BINDING CrmB PROTEIN DOMAIN AND Bv/G2R-dSECRET BACULOVIRUS DOMAIN PRODUCING SECRETING TNF-BINDING CrmB PROTEIN OF SMALLPOX VIRUS WITH DELETED SECRET DOMAIN |
2011 |
- Tregubchak Tat'Jana Vladimirovna
- Nepomnjashchikh Tat'Jana Sergeevna
- Antonets Denis Viktorovich
- Shchelkunov Sergej Nikolaevich
|
RU2471869C1 |
| METHOD FOR EXTRACTING AND PURIFYING RECOMBINANT PROTEIN, AN ANALOGUE OF A HUMAN KAPPA-CASEIN FRAGMENT HAVING CYTOTOXIC ACTIVITY TOWARDS HUMAN CANCER CELLS |
2018 |
- Chinak Olga Aleksandrovna
- Belovezhets Tatyana Nikolaevna
- Koval Olga Aleksandrovna
- Romanova Irina Vladimirovna
- Volkova Olga Yurevna
- Tkachenko Anastasiya Viktorovna
- Kuligina Elena Vladimirovna
- Taranin Aleksandr Vladimirovich
- Rikhter Vladimir Aleksandrovich
|
RU2693251C1 |
| RECOMBINANT PLASMID DNA PET3.54, ENCODING POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR, AND STRAIN OF BACTERIA ESCHERICHIA COLI - PRODUCER OF POLYPEPTIDE FN3.54, INTERACTING WITH HUMAN TUMOUR NECROSIS FACTOR |
2013 |
- Shingarova Ljudmila Nikolaevna
- Petrovskaja Lada Evgen'Evna
- Krjukova Elena Aleksandrovna
- Boldyreva Elena Filippovna
- Dolgikh Dmitrij Aleksandrovich
- Kirpichnikov Mikhail Petrovich
|
RU2531547C1 |
| METHOD FOR OBTAINING ACTIVE STARTING OF VACCINE AGAINST ROTAVIRUS INFECTION OF PROTEIN FLiCVP6VP8 |
2017 |
- Bogomolova Elena Grigorevna
- Dukhovlinov Ilya Vladimirovich
- Dobrovolskaya Olga Andreevna
- Fedorova Ekaterina Alekseevna
- Ishchuk Sergej Aleksandrovich
- Simbirtsev Andrej Semenovich
|
RU2649132C1 |
