METHOD OF PRODUCTION OF VACCINE FOR ANIMAL NECROBACTERIOSIS PREVENTION AND TREATMENT, VACCINE FOR ANIMAL NECROBACTERIOSIS PREVENTION AND TREATMENT AND METHOD OF ANIMAL NECROBACTERIOSIS PREVENTION AND TREATMENT Russian patent published in 2008 - IPC A61K39/02 A61P31/04 

FIELD: medicine.

SUBSTANCE: group of inventions concerns veterinary microbiology and immunology. Method of vaccine production includes cultivation of strain B.necrophomm "0-1" "ВИЭВ" on nutrient medium, inactivation with formalin, biomass isolation from culture fluid by centrifugation, centrifugate isolation and exotoxin ultrafiltration concentration to protein content 5.5-6.0 mg/ml (by Lowry). Exotoxin B.necrophomm of molecular weight 18000-20000 D is produced. It is added with formalin solution to end concentration 0.4% and inactivated within 15 days. Formalin inactivated exotoxin is mixed with oily adjuvant at the following component proportions (mass%): formalin inactivated exotoxin B.necrophorum - 60.0-67.0, oily adjuvant - 33.0-40.0. Then produced suspension is homogenised and end vaccine is packed up. A vaccine is injected intradermally in single or twice dosage 0.2-0.4 ml. Invention enables to simplify method of production of highly effective vaccine, to produce vaccine of reactogenity, as well as to lower vaccination dose and increase convenience of introduction.

EFFECT: development of effective method of necrobacteriosis vaccination in veterinary science.

10 cl, 4 ex