VACCINES FOR PREVENTION OF SPLENIC FEVER AND NECROTIC STOMATITIS IN ANIMALS AND METHOD FOR ITS PREPARATION Russian patent published in 2014 - IPC A61K39/116 A61K39/07 

FIELD: veterinary medicine.

SUBSTANCE: presented vaccine comprises the formalin-inactivated leukocidin-exotoxin obtained from production strain Fusobacterium necrophorum "0-1" Russian Research Institute of Experimental Veterinary Medicine ,1-10% inactivated bacterial mass of the said strain containing 7.0-7.7 billion cells per 1 cm³, glycerol and a mixture of mineral oil "Markol-52" and emulsifier taken in weight ratios of 1:(0.9-1.1), and also the suspension of live spores of splenic fever vaccine strain 55 Russian Research Institute of Veterinary Virology and Microbiology in saline, and adjuvant. The method of production of the vaccine comprises cultivation of the strain Fusobacterium necrophorum "0-1" RRIEVM, its inactivation with formalin, isolation of bacterial mass from the culture medium by centrifugation, isolation from the culture medium of exotoxin - leukocidin, its purification and concentration by ultrafiltration on hollow fibres, adding the suspension of live spores of the splenic fever vaccine strain 55-RRIVVM and saponin, and adding of 1-10% inactivated bacterial mass of strain Fusobacterium necrophorum "0-1" RRIEVM, glycerine and mixture of mineral oil "Markol-52" and emulsifier.

EFFECT: invention enables to obtain vaccine that provides sustained immunity.

3 cl, 7 ex, 1 tbl