GENETICALLY CONSTRUCTED DEPENDENT ON PYRROLOQUINOLINQUINONE GLUCOSODEHYDROGENASE, CONTAINING INSERTION OF AMINO ACID Russian patent published in 2008 - IPC C12N9/04 C12Q1/00 G01N33/66 C12N15/10 C12N15/53 C12M1/40 

FIELD: chemistry.

SUBSTANCE: invention relates to biotechnology. Version of solvable dependent on pyrroloquinolinquinone (PQQ) glucosodehydrogenase (s-GDH), containing insertion of amino acid residual between positions 428 and 429, which correspond to amino acid sequence of wild type known for Acinetobacter calcoaceticus and can contain one or several additional amino acid substitutions, is described. Represented is polynucleotide, coding described s-GDH version. Expression vector is described. It containes represented polynucleotide. Host-cell E.coli, containing described vector is described. Method of obtaining said s-GDH version, including cultivation of host-cell E.coli in conditions, suitable for obtaining enzyme version is described. Method of detection is described. It determines or measuring glucose in sample by means of described s-GDH version, which includes bringing sample in contact with said version.

EFFECT: obtaining possibility to determine concentration of sugars, especially glucose in sample efficiently.

20 cl, 8 ex, 4 dwg, 1 tbl