FIELD: biotechnology, in particular method for construction and production of mutant transglutaminases (MTG).
SUBSTANCE: invention relates to method for construction and production of mutant transglutaminases based on space structure of transglutaminase obtained from Streptoverticillium mobaraense, as well as to mutant MTG obtained by said method. Also disclosed are method for MTG modification based on space structure and modified transglutaminase with enhanced reactivity relative to substrate. Method of present invention makes it possible to predict MTG binding site to substrate on the base of space structure that is determined by MNG crystal X-ray analysis, and to design mutant transglutaminases by replacement, insertion or deletion of amino acid residues disposed on transglutaminase substrate-binding site.
EFFECT: new method for production and modification of mutant transglutaminases.
6 cl, 60 dwg, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PRODUCING MICROBIAL TRANSGLUTAMINASE | 2004 |
|
RU2316594C2 |
| METHOD FOR SECRETORY PROTEIN PRODUCTION | 2002 |
|
RU2264463C2 |
| GEN OF PHOSPHATIDIC ACID PHOSPHATASE | 2012 |
|
RU2625025C2 |
| GENETICALLY CONSTRUCTED DEPENDENT ON PYRROLOQUINOLINQUINONE GLUCOSODEHYDROGENASE, CONTAINING INSERTION OF AMINO ACID | 2005 |
|
RU2336306C1 |
| RECOMBINANT PRODUCER OF HUMAN OMEGA-AMIDASE Nit2 | 2021 |
|
RU2778559C1 |
| IMPROVED NITRILE HYDRATASE | 2015 |
|
RU2689606C2 |
| BINDING DOMAIN FOR BACTERIAL ATP-SYNTHASE | 2005 |
|
RU2418001C2 |
| RECOMBINANT ATTENUATED MICROORGANISMS CLOSTRIDIUM AND VACCINE | 2007 |
|
RU2445364C2 |
| METHOD TO PRODUCE L-AMINO ACID OF GLUTAMATE FAMILY USING CORYNEFORMIC BACTERIUM | 2011 |
|
RU2496867C2 |
| MUTANTS OF ACTIVATION INDUCED CYTIDINE DEAMINASE (AID) AND METHODS OF APPLYING THEREOF | 2010 |
|
RU2537264C2 |
