BIOREACTOR WITH EXPOSURE IN LIQUID AND GAS PHASES FOR CULTIVATION OF CELLS Russian patent published in 2008 - IPC C12M3/06 

FIELD: technological processes; chemistry.

SUBSTANCE: cells to be cultivated are located in hollow-fiber membranes and are alternately introduced into liquid nutrient medium and gas phase located in it. Device is a bioreactor with exposure in liquid and gas phases, which contains supply space, in which hollow-fiber membranes are located with internal diameter of not more than 5 mm, internal volumes of which form chambers for cultivation. After cells are introduced into chambers for cultivation, approximately half of supply space is filled with nutrient medium, and the other half is filled with gas phase. After connection of medium and gas perfusion, cyclical exposure of hollow-fiber membranes and cells located in them in gas and liquid phase starts.

EFFECT: possibility of efficient continuous cultivation of cells with high density and preparation of products from these cells at their simultaneous retention.

18 cl, 3 dwg