METHOD FOR DETACHABLE-TOPPING FERMENTATION WITH HIGH CELL DENSITY Russian patent published in 2020 - IPC C12P21/02 C12N5/02 C12M1/12 C12M3/06 

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. Disclosed is a method of producing a product selected from a biopolymer expressed by a cell, a cell and a microorganism in a bioreactor system with detachable-top fermentation. Method involves fermentation of cells expressing a biopolymer, cells or microorganism in a culture vessel. During fermentation, the impurity-containing medium is removed from the culture vessel through the filtering impurity element, adding a first fresh medium for replacement or partial replacement of the removed medium, certain volume of medium containing a product and admixtures is removed from the culture vessel through the product collection outlet and a second fresh medium is added to the culture vessel for replacement or partial replacement of the removed medium.

EFFECT: invention ensures increase in the bioreactor productivity and concentration of the product in the collected medium.

15 cl, 1 dwg