FIELD: biochemistry.
SUBSTANCE: invention relates to biochemistry. Disclosed is a method of increasing cell-specific productivity of recombinant factor VIII (rFVIII) produced in an eukaryotic cell suspension during culturing of said eucaryotic cell suspension in a culturing medium. Method involves culturing a cell suspension obtained by inducing mechanical shearing force on mammalian cell suspension culture by applying specific power of 3 W/m3. Culturing medium contains not more than 500 mcM CaCl2, non-ionic detergent.
EFFECT: invention provides high output of protein produced by cultured cells.
14 cl, 5 dwg, 2 tbl, 4 ex
| Title |
Year |
Author |
Number |
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2000 |
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- Mundt Vol'Fgang
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- Grill'Berger Leopol'D
- Mitterer Artur
|
RU2380412C2 |
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2009 |
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|
RU2536244C2 |
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2000 |
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- Mundt Vol'Fgang
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- Grill'Berger Leopol'D
- Mitterer Artur
|
RU2266325C2 |
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2008 |
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|
RU2477318C2 |
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1999 |
|
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2009 |
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|
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2005 |
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|
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2009 |
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|
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2015 |
|
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2012 |
- Grigor'Eva Ol'Ga Vasil'Evna
- Zaval'Nyj Mikhail Aleksandrovich
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|
RU2500818C1 |
