METHOD OF OBTAINING AVIDIN AND LYSOZYME Russian patent published in 2008 - IPC C12N9/36 

FIELD: chemistry; biochemistry.

SUBSTANCE: present invention pertains to biochemistry. To obtain lysozyme enzyme and avidin protein from albumen, chromatography of albumen homogenisate is carried out on silochrome C-80 at neutral pH (7.0-7.5). Ballast proteins are removed in a 0.2 M glycine - NaOH buffer in the presence of 0.3 M NaCl at pH 9.3-9.5. Target proteins are eluted together with carbonate buffer with 0.5-0.8 M NaCl, with pH 10.8-11.0, and subsequent separation of proteins through gel filtration on a column with sephadex G-75.

EFFECT: simplification of the method of obtaining electrophoretically pure proteins and increasing the efficiency of the method.

1 dwg, 4 ex