FIELD: chemistry; biochemistry.
SUBSTANCE: present invention pertains to biochemistry. To obtain lysostaphin protein, chromatographic purification of a culture liquid of Staphylococcus simulans biovar staphylolyticus is carried out on silochrome C-80 at neutral pH 7.0-7.5. Ballast proteins and coloured impurities are removed in 0.2 M glycine - NaOH buffer in the presence of 0.2 M NaCl at pH 9.0-9.3. Elution of the lysostaphin at pH 9.8-10.0 is carried out using a mixture of 0.4 M tris-HCl buffer and 0.2 M glycine - NaOH buffer 0.4 M NaCl.
EFFECT: increased output of electrophoretically pure enzyme and simplification of the process of obtaining it.
1 dwg, 1 tbl, 2 ex
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