FIELD: chemistry, biochemistry.
SUBSTANCE: invention relates to gene engineering and biotechnology and can be used in a pharmaceutical industry. Cleared and extracted polynucleotide encodes protein with functional activity of a glucose carrier and the GLUT4 protein amino acid lineage wherein valine in position 85 is replaced by methionine. The protein under consideration features biological activity in yeast strains S. cerevisiae deprived of functional carriers of glucose and Erg4 protein.
EFFECT: production of protein with functional activity of glucose carrier in yeasty expressing system.
5 cl, 3 tbl, 1 ex
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