POLYPEPTIDE HAVING NADH-DEPENDENT HMF-REDUCTASE ACTIVITY Russian patent published in 2013 - IPC C12N9/04 C12N15/53 C12N15/63 C12P7/10 

FIELD: biotechnologies.

SUBSTANCE: extracted polypeptide is provided, which has NADH-dependent HMF-reductase activity, where the specified polypeptide is by 80% homological by amino acid sequence SEQ ID NO:2, available in the description, which has replacements in positions corresponding to positions a) Y295C, b) Y295C and S117L, c) Y295C and S110P, or d) Y295C and S110P and S117L in SEQ ID NO:2. A nucleic acid is described, which codes the specified polypeptide, as well as an expression vector, containing the specified nucleic acid, and a host cell containing the specified vector and designed for expression of the polypeptide with NADH-dependent HMF-reductase activity. It is proposed to apply the specified polypeptide, nucleic acid, vector or host cell in production of large-capacity chemicals from lignocellulose raw materials and furane-containing material.

EFFECT: invention makes it possible to produce NADH-dependent HMF-reductase with increased specific activity.

18 cl, 6 tbl, 4 dwg, 7 ex