FIELD: chemistry, biochemistry.
SUBSTANCE: invention relates to biotechnology. The proposed method comprises thermal processing of larva, separated from a substratum upon termination of their cultivation, for 2 to 15 min at 75 to 100°C. Thermal processing over, larvae are crushed, chitine-containing fraction is separated from hemolymph. Now, the chitine-containing precipitate as-washed and crushed is subjected to enzymic hydrolysis at temperature 35 to 46°C with pH of 6.5 to 8.5 till achieving amine nitrogen content not below 0.8 G/%. Then, the separated chitine-containing precipitate is subjected to alkaline hydrolysis at continuous mixing for 60 to 90 min at 55 to 65°C. Finally, the aforesaid chitine-containing precipitate is subjected to demineralisation by 1.5 to 3.0 percent solution of hydrochloric or nitric acid for 30 to 40 min at 60 to 70°C. After that, the precipitate containing chitine is rinsed with water till pH of 6.5 to 7.5 and dried.
EFFECT: increase in biological value of target products with simultaneous increase in their number and simplification of process method.
4 cl, 1 ex
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Authors
Dates
2009-01-27—Published
2006-07-26—Filed