METHOD OF OBTAINING NATURAL AND MODIFIED DEOXYRIBO- AND RIBOOLIGONUCLEOTIDE 5'-TRIPHOSPHATE SALTS Russian patent published in 2009 - IPC C07H1/00 C07H21/02 

Abstract RU 2348643 C1

FIELD: chemistry.

SUBSTANCE: invention concerns method of obtaining natural and modified deoxy- and ribooligonucleotide 5'-triphosphate salts, involving monophosphation of initial reagent, protected natural or modified oligonucleotide of deoxy- or ribo- range in the form of 0.1-0.5 M solution, in pyridine by double or triple excess of phosphor oxychloride for 10-15 minutes; further processing of activated oligonucleotide derivative by tenfold to fifteenfold excess of pyrophosphate bis-tributylammonium salt solution in acetonitryl and 20-fold excess of tert-amine; reacting mix maturing for 15-30 minutes, followed by degradation of intermediary oligonucleotide trimetaphosphate derivative by triethylammonium bicarbonate buffer, purification of target product by reverse-phase chromatography (RPC), removal of protection groups from functional oligonucleotide groups, and second purification of target product by RPC. Target product output after purification comprises 45-95%. Purity grade of obtained compounds by the data of high-precision electronic liquid chromatography and nuclear magnetic resonance spectrography is over 95%.

EFFECT: obtaining compounds applicable in biological molecular and genetic engineering research.

2 cl, 1 dwg, 2 tbl, 7 ex

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RU 2 348 643 C1

Authors

Abramova Tat'Jana Veniaminovna

Vasil'Eva Svetlana Viktorovna

Koroleva Ljudmila Sergeevna

Vlasov Valentin Viktorovich

Sil'Nikov Vladimir Nikolaevich

Dates

2009-03-10Published

2007-09-03Filed