METHOD OF ERYTHROCYTES REACTANCE DETERMINATION Russian patent published in 2009 - IPC G01N33/48 

FIELD: medicine.

SUBSTANCE: method relates to area of physiology and membranology, and can be used in diagnostics of functional full value of cells. The way includes stabilisation of blood by a heparin, washing up of erythrocytes, preparation of suspension of erythrocytes, thus influence by the changed medium in the form of a pharmacological blocker of calcium channels or a blocker β-adrenergic receptors on erythrocytes of blood is carried out within 15 minutes on air at a room temperature, prepare delutions in the ratio blood/incubatory medium 1:50, and the first delution in 0.2% solution NaCL, and the second - in a 0.65% NaCL solution , incubate delutions within an hour and define morphometric indexes of erythrocytes. Then factor of a reserve surface of erythrocytes of blood for each time interval ΔT_K is defined. For every ΔT_K quantity of functionally defective erythrocytes for which the factor of a reserve surface is more than unit is defined. Time functional dependence (GΔT_K) reactivities of erythrocytes of blood by calculation GΔT_K for every ΔT_K is built, and reactance of erythrocytes of blood define as size of a time interval of influence on it medium with the changed structure after which efflux parity GΔT_K not less than 0.15.

EFFECT: working out of the simple way, allowing observing of a condition of live erythrocytes of blood, to select a dose of activators or blockers of membranous receptors with the least damaging activity.

4 cl, 2 ex, 2 dwg